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Vacuum autoclave cycle

On the other hand, when air cannot be easily removed from the sterilization medium, it is better to use vacuum autoclaving or vacuum pre-autoclaving. Items it typically sterilizes include animal cages and bedding or wrapped surgical kits.  
Autoclaves designed to perform vacuum cycles have a vacuum system. The system alternates steam injection and evacuation at the beginning of each cycle. This helps remove air from the autoclave chamber.  
Vacuum suction helps to completely remove air from the most difficult places to reach objects. Once all the air is gone, the steam will be able to penetrate the object quickly, allowing for a fast and efficient sterilization cycle.

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